Distance-dependent Variation with the Length of Dna Spacers in Fluorescence Signals of Dna-cy3-tailored Gold Nanoparticles Deposited on a Solid Surface
نویسندگان
چکیده
We report the distance-dependent fluorescence behavior of Au-DNA conjugates prepared by equilibrating the phosphine-stabilized gold nanoparticles (AuNPs) of 10-nm size with the designed rigid spacer double-stranded DNA consisting of thiol-modified target and Cy3-labelled complementary probe of different lengths (5 to 20 nm). The products were immobilized onto a MPTMS (3-mercaptopropyltrimethoxysilane)-modified glass substrate and were finally excited with a 532-nm laser source. Fluorescence quenching in both solution and solid platforms was assessed via disulfide bond cleavage between Au and DNA-Cy3 using mercaptoethanol. Successful and effective cleavage revealed by UV-vis absorption and fluorescence profiles was achieved in just only a few minutes.
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